Infectious bronchitis virus generates spherules from zippered endoplasmic reticulum membranes.
Identifieur interne : 001915 ( Main/Exploration ); précédent : 001914; suivant : 001916Infectious bronchitis virus generates spherules from zippered endoplasmic reticulum membranes.
Auteurs : Helena J. Maier [Royaume-Uni] ; Philippa C. Hawes ; Eleanor M. Cottam ; Judith Mantell ; Paul Verkade ; Paul Monaghan ; Tom Wileman ; Paul BrittonSource :
- mBio [ 2150-7511 ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux, Humains, Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (virologie), Lignée cellulaire, Maladies de la volaille (virologie), Membranes intracellulaires (), Membranes intracellulaires (ultrastructure), Membranes intracellulaires (virologie), Poulets, Réplication virale, Réticulum endoplasmique (), Réticulum endoplasmique (ultrastructure), Réticulum endoplasmique (virologie), Virus de la bronchite infectieuse (génétique), Virus de la bronchite infectieuse (physiologie).
- MESH :
- génétique : Virus de la bronchite infectieuse.
- médecine vétérinaire : Infections à coronavirus.
- physiologie : Virus de la bronchite infectieuse.
- virologie : Infections à coronavirus, Maladies de la volaille, Membranes intracellulaires, Réticulum endoplasmique.
- Animaux, Humains, Lignée cellulaire, Membranes intracellulaires, Poulets, Réplication virale, Réticulum endoplasmique.
English descriptors
- KwdEn :
- Animals, Cell Line, Chickens, Coronavirus Infections (veterinary), Coronavirus Infections (virology), Endoplasmic Reticulum (chemistry), Endoplasmic Reticulum (ultrastructure), Endoplasmic Reticulum (virology), Humans, Infectious bronchitis virus (genetics), Infectious bronchitis virus (physiology), Intracellular Membranes (chemistry), Intracellular Membranes (ultrastructure), Intracellular Membranes (virology), Poultry Diseases (virology), Virus Replication.
- MESH :
- chemistry : Endoplasmic Reticulum, Intracellular Membranes.
- genetics : Infectious bronchitis virus.
- physiology : Infectious bronchitis virus.
- ultrastructure : Endoplasmic Reticulum, Intracellular Membranes.
- veterinary : Coronavirus Infections.
- virology : Coronavirus Infections, Endoplasmic Reticulum, Intracellular Membranes, Poultry Diseases.
- Animals, Cell Line, Chickens, Humans, Virus Replication.
Abstract
Replication of positive-sense RNA viruses is associated with the rearrangement of cellular membranes. Previous work on the infection of tissue culture cell lines with the betacoronaviruses mouse hepatitis virus and severe acute respiratory syndrome coronavirus (SARS-CoV) showed that they generate double-membrane vesicles (DMVs) and convoluted membranes as part of a reticular membrane network. Here we describe a detailed study of the membrane rearrangements induced by the avian gammacoronavirus infectious bronchitis virus (IBV) in a mammalian cell line but also in primary avian cells and in epithelial cells of ex vivo tracheal organ cultures. In all cell types, structures novel to IBV infection were identified that we have termed zippered endoplasmic reticulum (ER) and spherules. Zippered ER lacked luminal space, suggesting zippering of ER cisternae, while spherules appeared as uniform invaginations of zippered ER. Electron tomography showed that IBV-induced spherules are tethered to the zippered ER and that there is a channel connecting the interior of the spherule with the cytoplasm, a feature thought to be necessary for sites of RNA synthesis but not seen previously for membrane rearrangements induced by coronaviruses. We also identified DMVs in IBV-infected cells that were observed as single individual DMVs or were connected to the ER via their outer membrane but not to the zippered ER. Interestingly, IBV-induced spherules strongly resemble confirmed sites of RNA synthesis for alphaviruses, nodaviruses, and bromoviruses, which may indicate similar strategies of IBV and these diverse viruses for the assembly of RNA replication complexes.
DOI: 10.1128/mBio.00801-13
PubMed: 24149513
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001111
- to stream PubMed, to step Curation: 001111
- to stream PubMed, to step Checkpoint: 001155
- to stream Ncbi, to step Merge: 002769
- to stream Ncbi, to step Curation: 002769
- to stream Ncbi, to step Checkpoint: 002769
- to stream Main, to step Merge: 001922
- to stream Main, to step Curation: 001915
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Infectious bronchitis virus generates spherules from zippered endoplasmic reticulum membranes.</title><author><name sortKey="Maier, Helena J" sort="Maier, Helena J" uniqKey="Maier H" first="Helena J" last="Maier">Helena J. Maier</name><affiliation wicri:level="1"><nlm:affiliation>The Pirbright Institute, Compton Laboratory, Compton, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Pirbright Institute, Compton Laboratory, Compton</wicri:regionArea><wicri:noRegion>Compton</wicri:noRegion></affiliation></author><author><name sortKey="Hawes, Philippa C" sort="Hawes, Philippa C" uniqKey="Hawes P" first="Philippa C" last="Hawes">Philippa C. Hawes</name></author><author><name sortKey="Cottam, Eleanor M" sort="Cottam, Eleanor M" uniqKey="Cottam E" first="Eleanor M" last="Cottam">Eleanor M. Cottam</name></author><author><name sortKey="Mantell, Judith" sort="Mantell, Judith" uniqKey="Mantell J" first="Judith" last="Mantell">Judith Mantell</name></author><author><name sortKey="Verkade, Paul" sort="Verkade, Paul" uniqKey="Verkade P" first="Paul" last="Verkade">Paul Verkade</name></author><author><name sortKey="Monaghan, Paul" sort="Monaghan, Paul" uniqKey="Monaghan P" first="Paul" last="Monaghan">Paul Monaghan</name></author><author><name sortKey="Wileman, Tom" sort="Wileman, Tom" uniqKey="Wileman T" first="Tom" last="Wileman">Tom Wileman</name></author><author><name sortKey="Britton, Paul" sort="Britton, Paul" uniqKey="Britton P" first="Paul" last="Britton">Paul Britton</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:24149513</idno><idno type="pmid">24149513</idno><idno type="doi">10.1128/mBio.00801-13</idno><idno type="wicri:Area/PubMed/Corpus">001111</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001111</idno><idno type="wicri:Area/PubMed/Curation">001111</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001111</idno><idno type="wicri:Area/PubMed/Checkpoint">001155</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001155</idno><idno type="wicri:Area/Ncbi/Merge">002769</idno><idno type="wicri:Area/Ncbi/Curation">002769</idno><idno type="wicri:Area/Ncbi/Checkpoint">002769</idno><idno type="wicri:Area/Main/Merge">001922</idno><idno type="wicri:Area/Main/Curation">001915</idno><idno type="wicri:Area/Main/Exploration">001915</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Infectious bronchitis virus generates spherules from zippered endoplasmic reticulum membranes.</title><author><name sortKey="Maier, Helena J" sort="Maier, Helena J" uniqKey="Maier H" first="Helena J" last="Maier">Helena J. Maier</name><affiliation wicri:level="1"><nlm:affiliation>The Pirbright Institute, Compton Laboratory, Compton, United Kingdom.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>The Pirbright Institute, Compton Laboratory, Compton</wicri:regionArea><wicri:noRegion>Compton</wicri:noRegion></affiliation></author><author><name sortKey="Hawes, Philippa C" sort="Hawes, Philippa C" uniqKey="Hawes P" first="Philippa C" last="Hawes">Philippa C. Hawes</name></author><author><name sortKey="Cottam, Eleanor M" sort="Cottam, Eleanor M" uniqKey="Cottam E" first="Eleanor M" last="Cottam">Eleanor M. Cottam</name></author><author><name sortKey="Mantell, Judith" sort="Mantell, Judith" uniqKey="Mantell J" first="Judith" last="Mantell">Judith Mantell</name></author><author><name sortKey="Verkade, Paul" sort="Verkade, Paul" uniqKey="Verkade P" first="Paul" last="Verkade">Paul Verkade</name></author><author><name sortKey="Monaghan, Paul" sort="Monaghan, Paul" uniqKey="Monaghan P" first="Paul" last="Monaghan">Paul Monaghan</name></author><author><name sortKey="Wileman, Tom" sort="Wileman, Tom" uniqKey="Wileman T" first="Tom" last="Wileman">Tom Wileman</name></author><author><name sortKey="Britton, Paul" sort="Britton, Paul" uniqKey="Britton P" first="Paul" last="Britton">Paul Britton</name></author></analytic><series><title level="j">mBio</title><idno type="eISSN">2150-7511</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chickens</term><term>Coronavirus Infections (veterinary)</term><term>Coronavirus Infections (virology)</term><term>Endoplasmic Reticulum (chemistry)</term><term>Endoplasmic Reticulum (ultrastructure)</term><term>Endoplasmic Reticulum (virology)</term><term>Humans</term><term>Infectious bronchitis virus (genetics)</term><term>Infectious bronchitis virus (physiology)</term><term>Intracellular Membranes (chemistry)</term><term>Intracellular Membranes (ultrastructure)</term><term>Intracellular Membranes (virology)</term><term>Poultry Diseases (virology)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Infections à coronavirus (virologie)</term><term>Lignée cellulaire</term><term>Maladies de la volaille (virologie)</term><term>Membranes intracellulaires ()</term><term>Membranes intracellulaires (ultrastructure)</term><term>Membranes intracellulaires (virologie)</term><term>Poulets</term><term>Réplication virale</term><term>Réticulum endoplasmique ()</term><term>Réticulum endoplasmique (ultrastructure)</term><term>Réticulum endoplasmique (virologie)</term><term>Virus de la bronchite infectieuse (génétique)</term><term>Virus de la bronchite infectieuse (physiologie)</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Endoplasmic Reticulum</term><term>Intracellular Membranes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de la bronchite infectieuse</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la bronchite infectieuse</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Infectious bronchitis virus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Endoplasmic Reticulum</term><term>Intracellular Membranes</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term><term>Maladies de la volaille</term><term>Membranes intracellulaires</term><term>Réticulum endoplasmique</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Endoplasmic Reticulum</term><term>Intracellular Membranes</term><term>Poultry Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chickens</term><term>Humans</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Lignée cellulaire</term><term>Membranes intracellulaires</term><term>Poulets</term><term>Réplication virale</term><term>Réticulum endoplasmique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Replication of positive-sense RNA viruses is associated with the rearrangement of cellular membranes. Previous work on the infection of tissue culture cell lines with the betacoronaviruses mouse hepatitis virus and severe acute respiratory syndrome coronavirus (SARS-CoV) showed that they generate double-membrane vesicles (DMVs) and convoluted membranes as part of a reticular membrane network. Here we describe a detailed study of the membrane rearrangements induced by the avian gammacoronavirus infectious bronchitis virus (IBV) in a mammalian cell line but also in primary avian cells and in epithelial cells of ex vivo tracheal organ cultures. In all cell types, structures novel to IBV infection were identified that we have termed zippered endoplasmic reticulum (ER) and spherules. Zippered ER lacked luminal space, suggesting zippering of ER cisternae, while spherules appeared as uniform invaginations of zippered ER. Electron tomography showed that IBV-induced spherules are tethered to the zippered ER and that there is a channel connecting the interior of the spherule with the cytoplasm, a feature thought to be necessary for sites of RNA synthesis but not seen previously for membrane rearrangements induced by coronaviruses. We also identified DMVs in IBV-infected cells that were observed as single individual DMVs or were connected to the ER via their outer membrane but not to the zippered ER. Interestingly, IBV-induced spherules strongly resemble confirmed sites of RNA synthesis for alphaviruses, nodaviruses, and bromoviruses, which may indicate similar strategies of IBV and these diverse viruses for the assembly of RNA replication complexes.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><noCountry><name sortKey="Britton, Paul" sort="Britton, Paul" uniqKey="Britton P" first="Paul" last="Britton">Paul Britton</name><name sortKey="Cottam, Eleanor M" sort="Cottam, Eleanor M" uniqKey="Cottam E" first="Eleanor M" last="Cottam">Eleanor M. Cottam</name><name sortKey="Hawes, Philippa C" sort="Hawes, Philippa C" uniqKey="Hawes P" first="Philippa C" last="Hawes">Philippa C. Hawes</name><name sortKey="Mantell, Judith" sort="Mantell, Judith" uniqKey="Mantell J" first="Judith" last="Mantell">Judith Mantell</name><name sortKey="Monaghan, Paul" sort="Monaghan, Paul" uniqKey="Monaghan P" first="Paul" last="Monaghan">Paul Monaghan</name><name sortKey="Verkade, Paul" sort="Verkade, Paul" uniqKey="Verkade P" first="Paul" last="Verkade">Paul Verkade</name><name sortKey="Wileman, Tom" sort="Wileman, Tom" uniqKey="Wileman T" first="Tom" last="Wileman">Tom Wileman</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Maier, Helena J" sort="Maier, Helena J" uniqKey="Maier H" first="Helena J" last="Maier">Helena J. Maier</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001915 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001915 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24149513
|texte= Infectious bronchitis virus generates spherules from zippered endoplasmic reticulum membranes.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24149513" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |